El sueño en estudiantes de enfermería en una universidad del sur de Chile / Sleep in nursing students at a university in southern Chile

El sueño es una función y necesidad básica en el ser humano; permite el descanso de la mente y cuerpo, recuperar energías y restablecer funciones para un buen rendimiento. Los trastornos del sueño pueden desencadenar problemas gástricos, cardiovasculares, cognitivas, desequilibrio en el rendimiento académico, alterando negativamente la capacidad de concentración, aprendizaje, memoria; dormir poco se asocia a fatiga y disminución de las capacidades cognitivas y motoras durante el día. Las demandas sociales, familiares y académicas de estudiantes universitarios los hace adoptar patrones de sueño irregulares, dando lugar a cambios en el hábito del sueño, especialmente en el contexto de la pandemia por COVID-19, debido al incremento de horas de conexión tele presencial y el uso de aparatos electrónicos para sus actividades académicas. Por ello el presente estudio evaluado y aprobado por Comité De Ética, certificado ORD: Nº 145- 2021/RevOct.2021, pretende describir la calidad del sueño en estudiantes de Enfermería en una Universidad del sur de Chile durante el segundo semestre del 2021; aplicando el Índice de Calidad de Sueño de Pittsburgh a 165 estudiantes de primero a quinto año, a través de un estudio de tipo cuantitativo, observacional, descriptivo, de secuencia transversal, con muestreo probabilístico estratificado y con afijación simple. Solo el 12% de los estudiantes de Enfermería presentó óptima calidad del sueño; el 64% sugiere problemas de sueño y el 24% se acercan a una peor calidad de sueño. La existencia de una alta prevalencia de la mala calidad del sueño indica la necesidad de adquirir una buena higiene del sueño que permita el descanso adecuado y necesario para realizar sus actividades de manera óptima.

Sleep is a basic function and need in humans; it allows the brain and body to rest, recover energy, and restore functions for good performance. Sleep disorders can trigger gastric, cardiovascular, and cognitive problems, as well as affect academic performanceby negatively altering the ability to concentrate, learn, and recall. Insufficient sleep is associated with fatigue and decreased cognitive and motor skills during the day. The social, family, and academic demands of university students often make them adopt irregular sleep patterns, leading to changes in sleep habits. This was especially true during the COVID-19 pandemic, due to the increase in hours of online connection and the use of electronic devices for academic activities. This study, evaluated and approved by the Ethics Committee, certified ORD: Nº 145- 2021/RevOct.2021, aims to describe the quality of sleep in Nursing students at a university in southern Chile during the second semester of 2021. The Pittsburgh Sleep Quality Index was administered to 165 first-year to fifth-year Nursing students, through a quantitative, observational, descriptive, cross-sectional sequence study, with stratified probabilistic sampling and simple assignment. Only 12% of Nursing students had optimal sleep quality; 64% suggest sleep problems and 24% indicated a worse quality of sleep. The high prevalence of poor sleep quality indicates the need for students to adopt good sleep hygiene that allows adequate and necessary rest for optimal academic performance.

Systemic neosporosis in a dog treated for immune-mediated thrombocytopenia and hemolytic anemia.

A 4-year-old male Toy Poodle was presented to the Small Animal Veterinary Hospital of the Faculty of Veterinary Medicine of the Autonomous University of Mexico (FMVZ, UNAM) because of depression, lethargy, and hemorrhages involving several areas of the skin and around the eyes. Hematology data and a bone marrow analysis suggested hemolytic anemia and immune-mediated thrombocytopenia. The dog was treated with prednisone, and after one month the hematology variables improved. However, the dog's clinical condition inexplicably worsened and it was euthanized. On necropsy, there were no relevant findings. However, in histology, multifocal lymphoplasmacytic and histiocytic meningoencephalitis and necrosis, and a protozoan cyst in the cerebellum were identified. In addition, moderate multifocal lymphoplasmacytic and necrotizing pancreatitis, hepatitis, myocarditis, and diffuse lymphoplasmacytic enteritis were observed. Immunohistochemistry of the cerebellum, liver, pancreas, and intestine with a specific antibody against Neospora caninum confirmed the diagnosis of systemic neosporosis. The systemic neosporosis in this dog was most likely caused by reactivation of latent parasites due to prednisone administration during the one month of treatment. It should be kept in mind that in dogs being treated with immunosuppressants for immune-mediated conditions, opportunistic parasites, such as Toxoplasma gondii and N caninum, can be reactivated from a latent state, as it probably happened in the present case.

HIV medical providers' perceptions of the use of antiretroviral therapy as nonoccupational postexposure prophylaxis in 2 major metropolitan areas.

INTRODUCTION: In 2005, the Centers for Disease Control and Prevention expanded its recommendation of postexposure prophylaxis (PEP) use in the workplace to include nonoccupational exposures (nPEP). The availability and extensive use of nPEP have not achieved widespread acceptance among health-care providers of high-risk populations, and public health and primary care agencies have been sparse in their implementation of nPEP promotion, protocols, and practices. METHODS: We conducted a survey of HIV providers (n = 142, response rate = 61%) in Miami-Dade County (Florida) and the District of Columbia that focused on their knowledge, attitudes, beliefs, and practices related to the delivery of nPEP. We then analyzed differences in survey responses by site and by history of prescribing nPEP using bivariate and multivariate logistic regression. RESULTS: More District of Columbia providers (59.7%) reported ever prescribing nPEP than in Miami (39.5%, P < 0.048). The majority of practices in both cities did not have a written nPEP protocol and rarely or never had patients request nPEP. Multivariable analysis for history of prescribing nPEP was dominated by having patients request nPEP [odds ratio (OR) = 21.53] and the belief that nPEP would lead to antiretroviral resistance (OR = 0.14) and having a written nPEP protocol (OR = 7.49). DISCUSSION: Our findings are consistent with earlier studies showing the underuse of nPEP as a prevention strategy. The significance of having a written nPEP protocol and of patient requests for nPEP speaks to the importance of using targeted strategies to promote widespread awareness of the use of HIV antiretroviral medications as a prevention intervention.

Development of an ultra-performance liquid chromatography technique coupled with mass spectrometry for the measurement of tacrolimus in micro-samples of whole blood, and its application on a pharmacokiinetic trial.

OBJECTIVE: The aim was to develop a rapid, specific, sensitive and accurate chromatographic technique coupled with mass spectrometry for the measurement of tacrolimus (CAS 104987-11-3) in microsamples of whole blood, and its application on a pharmacokinetic pilot trial. METHODS: A fast gradient was designed in an ultra-performance liquid chromatography, and coupled with a mass spectrometer for the quantification of tacrolimus in 100 microl samples of EDTA whole blood. Multiple reaction monitoring was used for the measurement of tacrolimus (m/z(+1) 821.49-->4768.35 Th) and sirolimus as internal standard (m/z(+1) 931.69-->864.39 Th). The method was validated according to Mexican regulatory guidelines. Twenty-four young healthy male volunteers with similar hematocrit values participated in the pharmacokinetic trial; an oral single dose of one 5 mg tacrolimus capsule was administered and kinetic profiles were described since 0 h until 24 h post-dose. RESULTS: Method showed to be accurate, precise and linear over the range from 1 to 80 ng/ml, having an absolute recovery of 94%. Molecule was stable for two months at -70 degrees C, and heparin interfered with its quantification. Total run-time is around 1.5 min. Mean maximum blood concentration was 32.63 +/- 1.74 ng/ml, and was reached at 1 h post-dose; elimination half-life was 14.18 +/- 5.71 h. CONCLUSIONS: Method developed is not time-consuming, inexpensive, and sensitive enough for its application during pharmacokinetic trials, and can be suitable for therapeutic drug monitoring in transplanted patients. Pharmacokinetic data obtained in Mexican population are quite similar to previously reported in international literature.

Estudio invitro del efecto antimicrobiano de un cemento endodóntico sobre microorganismos anaerobicos comunes en conductos necróticos / In Vitro evaluation of antimicrobial effect of an endodontic cement om common anaerobic microorganisms in necrotic root canals

Este estudio se realizó con el fin de evaluar el efecto antimicrobiano de la "Pasta Proquident " sobre los microorganismos anaerobios estrictos más frecuentes en conductos radiculares necróticos.- Se utilizaron los métodos de difusión en agar y dilución en caldo. Las bacterias seleccionadas fueron: Prevotella intermedia, Porphyromona endodontalis, Peptostreptococcus micros, Fusobacterium nucleatum, Porphyromona gingivalis y Prevotella oralis. Se hicieron lecturas a los tres, seis y nueve días en el método de difusión en agar y a los dos, cuatro y seis días en el método de dilución en caldo.- Se encontró que la "Pasta Proquident tiene efecto bactericida sobre los gérmenes anaerobios estudiados y que tal efecto se prolongó durante el tiempo de observación. Esto significa que el cemento no pierde su efecto antibacteriano después del fraguado.